Cystatin A, a mammalian cysteine proteinase inhibitor

mechanism of inhibition of target proteinases by recombinant cystatin A variants by Sergio Estrada

Publisher: Sveriges Lantbruksuniversitet in Uppsala

Written in English
Published: Downloads: 921
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  • Cysteine proteinases.,
  • Proteinase.,
  • Recombinant proteins.

Edition Notes

Series of papers combined to form a doctoral thesis for the Swedish University of Agricultural Sciences, Uppsala.

StatementSergio Estrada.
SeriesActa Universitatis Agriculturae Sueciae -- 39
ContributionsSveriges Lantbruksuniversitet.
The Physical Object
Paginationvarious pagings :
ID Numbers
Open LibraryOL22415766M
ISBN 109157654484

Cystatin superfamily of proteinase inhibitors. New York: Nova Biomedical Books, © (DLC) (OCoLC) Material Type: Document, Internet resource cystatins, and kininogens -- The cystatin superfamily -- Structure: chicken cystatin and human cystatin C -- Cysteine proteinase inhibitors -- Dimerization and domain swapping. @article{osti_, title = {Bmcystatin, a cysteine proteinase inhibitor characterized from the tick Boophilus microplus}, author = {Lima, Cassia A and Sasaki, Sergio D and Tanaka, Aparecida S}, abstractNote = {The bovine tick Rhipicephalus (Boophilus) microplus is a blood-sucking animal, which is responsible for Babesia spp and Anaplasma marginale transmission for cattle. Jian Ni, Magnus Abrahamson, Mei Zhang, Marcia Alvarez Fernandez, Anders Grubb, Jeffrey Su, Guo-Liang Yu, Yuling Li, David Parmelee, Lily Xing, Timothy A. Coleman.   A barley cDNA clone encoding a putative cysteine protease with sequence homology to cathepsin B from mammalian cells has been characterized. This barley gene (CatB) is ubiquitously expressed, its mRNA being detected in leaves and roots, immature, mature and germinating embryos, in developing endosperms, and in aleurones upon germination, as.

To this end, we transformed soybean cells with constructs carrying genes encoding three different protease inhibitors: (1) Sbcys soybean cystatin, which is a specific cysteine protease inhibitor (Hines et al., ); (2) Kunitz, an inhibitor of the trypsin-like proteases (Kido et al., ); and (3) CII, a Bowman-Birk–type inhibitor that is. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): Purpose: Cystatin C is a mammalian cysteine protease inhibitor. This study describes the localization of cystatin C in the anterior segment of normal rat and mouse eyes. Cysteine proteases play an important role in protein degradation (e.g. of photoreceptor outer segments in the retinal pigment epithelium) and the. Cystatins are efficient inhibitors of papain-like cysteine proteinases, and they serve various important physiological functions. In this study, a novel cystatin, Cystatin-X, was cloned from a cDNA library of the skin of Bufo single nonglycosylated polypeptide chain of Cystatin-X consisted of amino acid residues, including seven cysteines. Cystatin C is a cysteine (thiol) protease inhibitor that belongs to the type II cystatin gene superfamily and is the most abundant extracellular inhibitor of cysteine proteases. Cystatin C is a constitutively secreted, amyloidogenic protein, which forms a two-fold symmetric dimer and modulates both cysteine protease activity and the expression.

  Cysteine proteinase inhibitors of the cystatin superfamily have several important functions in plants, including the inhibition of exogenous cysteine proteinases during herbivory or infection. Here we used a maximum‐likelihood approach to assess whether plant cystatins, like other proteins implicated in host–pest interactions, have been. Cysteine Proteinase Inhibitors Stefin A, Stefin B, and Cystatin C in Sera from Patients with Colorectal Cancer: Relation to Prognosis1 Nature Biotechnol - () doi/ The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic. The major inhibitor of the cysteine class of proteinases found in human body fluids, such as spinal fluid, milk, and seminal plasma, is cystatin C. In this study we show that human bronchoalveolar fluid also contains cystatin C and examine cystatin C expression by alveolar macrophages in vitro. Immunoprecipitation of extracts of metabolically labeled cells and immunoblotting of cellular. Cysteine proteinases and cystatin C in bronchoalveolar lavage fluid from subjects with subclinical emphysema K. Takeyabu, T. Betsuyaku, M. Nishimura, A. Yoshioka, M. Tanino, K. Miyamoto, Y. Kawakami aa Protease–antiprotease imbalance theory has been a cen-tral theme for the last 30 years in the study of the patho-.

Cystatin A, a mammalian cysteine proteinase inhibitor by Sergio Estrada Download PDF EPUB FB2

69, 76 C13 cysteine protease involved in antigen pocessing, mammalian legumain or asparaginyl endopeptidase (AEP), is also inhibited by cystatin F although it showes reduced affinity for AEP compared with cystatins C and E/M.

63 The inhibitor is expressed selectively in immune cells such as cytotoxic T cells, natural killer cells (NK cells Cited by: Cystatin-A is a protein that in humans is encoded by the CSTA gene. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences.

Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory s: CSTA, AREI, STF1, STFA, Cystatin A, PSS4. Cystatins are the thiol Proteinase inhibitors, present ubiquitously in mammalian body.

They prevent unwanted proteolysis and play important role in several diseases. Regulation of cysteine Proteinase and their inhibitors is of utmost importance in neurodegenerative diseases like Alzheimer, amyloid a Cited by: 1.

The cystatins are a family of cysteine protease inhibitors which share a sequence homology and a common tertiary structure of an alpha helix lying on top of an anti-parallel beta sheet.

The family is subdivided as described below. Cystatins show similarity to fetuins, kininogens, histidine-rich glycoproteins and cystatin-related proteins.

Cystatins mainly inhibit peptidase enzymes (another InterPro: IPR Cystatins belong to a large superfamily of cysteine protease inhibitors. They generally contain – amino acids, and their fold is composed of a five-stranded β sheet and an α helix that is packed orthogonally onto the β strands.

42 The substrate binding sites of cystatins are highly conserved and located Cystatin A β hairpin loops that connect strands 2 and 3 (site I) and 4 and 5 (site II. The cystatin superfamily of proteins, derived Cystatin A a common ancestor, is comprised of a diverse group of potent cysteine proteinase inhibitors and antibacterial/viral agents grouped into several families.

Cystatin A (acid cysteine proteinase inhibitor; ACPI) is a natural inhibitor of cysteine proteinases. It has been suggested that an inverse correlation exists between cystatin A and malignant progression. We wanted to assess the biological and clinical significance of cystatin A in infiltrative breast carcinoma by immunohistochemical staining.

Formalin-fixed paraffin-embedded material from Cystatin protease inhibitors and immune functions Article Literature Review (PDF Available) in Frontiers in Bioscience 13(12) February with Reads How we measure 'reads'.

Cystatin S: a cysteine proteinase inhibitor of human saliva. Isemura S, Saitoh E, Ito S, Isemura M, Sanada K. An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially.

Cystatin A (acid cysteine proteinase inhibitor; ACPI) is a natural inhibitor of cysteine proteinases. It has been suggested that an inverse correlation exists between cystatin A and malignant progression.

We wanted to assess the biological and clinical significance of cystatin A in infiltrative breast carcinoma by immunohistochemical staining. For. Cystatin-C is a small protein ( aminoacid, kDa), a cysteine proteinase inhibitor, product of a “housekeeping” gene expressed in all nuclear cells.

6 Cystatine-C has many characteristics of an ideal marker of renal function, it has constant rate of production and is freely filtrated by the glomerulus. 6,7 Although it is not secreted by. In mammalian cells cystatin M may target any of the lysosomal cathepsins B, L, H, and S or an unidentified cysteine proteinase with papain-like activity.

Unlike serine proteases, cystatins do not bind covalently to target proteinases but rather block their active site and display a broad specificity. Cysteine proteinase inhibitor Cystatin A in breast cancer Article (PDF Available) in Cancer Research 58(3) March with 65 Reads How we measure 'reads'.

At least for lysosomal cathepsins of mammalian cells, there are indications that their activity is regulated by endogenous cysteine protease inhibitors from the cystatin superfamily (Rawlings and Barrett, ). For example, cystatin C regulates cell surface expression of MHC class II molecules in dendritic cells (Pierre and Mellman, ).

The name cystatin was first used by Barrett [28] to describe an inhibitor that had been discovered and par- tially characterized from chicken egg-white of papain, ficin and other related cysteine endopeptidases [29]. When other protein inhibitors of cysteine proteinases.

Abstract. Cystatin C is a low molecular weight cationic protein produced by all nucleated cells which is a potent cysteine protease inhibitor. Its plasma concentration is proportional with glomerular filtration as it is synthesized at a constant rate, freely filtered through the glomerulus, and largely reabsorbed and catabolized in the proximal renal tubule with no tubular secretion which.

The single Trp of human cystatin C, Trp, is located in the second hairpin loop of the proteinase binding surface. Substitution of this residue by Gly markedly altered the spectroscopic changes accompanying papain binding and reduced the affinity for papain, actinidin, and cathepsins B and H by −fold.

The decrease in affinity indicated that the side chain of Trp contributes a. We aimed to assess the biological and clinical significance of the human cysteine protease inhibitor cystatin M/E, encoded by the CTS6 gene, in diseases of human hair and skin.

Exome and Sanger. The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH and 25 °C by 1H and 15N NMR spectroscopy.

The main body (Glu13−Asp97) of oryzacystatin-I is well-defined and consists of an α-helix and a five-stranded antiparallel β-sheet, while the N- and C-terminal regions (Ser2−Val Cystatin C is the most thoroughly studied mammalian cystatin, being the most abundant extracellular inhibitor of cysteine proteases.

It was originally described in by Grubb and Löfberg [14]. The gene encoding the protein is located in cystatin multigene locus on chromosome Mature human cystatin C is composed of amino acid. In plants, cystatins are natural and specific inhibitors of cysteine proteases of the papain C1A family that generally block C1A proteases by a tight and reversible interaction [].Several cystatin functions have been proposed, but all involve a balanced interplay with a cysteine protease to regulate proteolytic activity [],[].Research has so far provided strong evidence that plant cystatins.

A cDNA encoding a cysteine protease inhibitor (cystatin) was identified by immunoscreening a Haemonchus contortus cDNA library with antisera from lambs vaccinated with a protective membrane protein complex (H-gal-GP) derived from the gut of the parasite.

The cDNA sequence, designated Cys-1, showed significant levels of similarity with cystatins from several species of nematode as well as with. Cystatin E is thus a functional cysteine proteinase inhibitor despite relatively low amino acid sequence similarities with human cystatins (% identity with sequences for the Family 2 cystatins C, D, S, SN, and SA; 30% with the Family 1 cystatins, A and B, and domains 2 and 3 of the Family 3 cystatin, kininogen).

Unlike other human low Mr. Title: Binding Energetics Of The Inhibitor Cystatin To The Cysteine Proteinase Actinidin VOLUME: 10 ISSUE: 2 Author(s):Maricela Neria-Rios, Jaqueline Padilla-Zuniga, Enrique Garcia-Hernandez, Salvador R.

Tello-Solis and Rafael A. Zubillaga Affiliation:Departamento de Quimica, Universidad Autonoma Metropolitana Iztapalapa, A.P.Mexico D.F.,Mexico AIMS: Increased or altered activities of cysteine proteases have been implicated in serious human disorders such as cancer, rheumatoid arthritis, sepsis, and osteoporosis.

To improve the current knowledge of the regulatory role of a major mammalian cysteine protease inhibitor, cystatin C, in such disease processes, a cystatin C deficient mouse was generated and characterized. Aims: To purify and characterize Thiol protease inhibitor from buffalo brain and to compare its properties with respect to tissue and organ difference from other mammalian cystatins.

Main methods: Inhibitor has been isolated and purified using alkaline treatment; ammonium sulphate fractionation and gel filtration chromatography on Sephadex G family with endogenous inhibitors of cysteine proteases such as catepsins B, H and LAcid cysteine proteinase inhibitor (ACPI cystatin A) was the first identified mammalian cystatin, originally purified and biochemically characterised from rat skin.5 Furthermore, it has also been demonstrated in other.

The endogenous cysteine protease inhibitors represent the final level at which cysteine protease activity can be regulated. These inhibitors are subdivided into three families (stefins, cystatins and kininogens) which belong to the protein superfamily, cystatins.

Cystatins do not form a covalent bon. Cystatin superfamily of proteinase inhibitors. New York: Nova Biomedical Books, © (OCoLC) Document Type: Book: All Authors / Contributors: cystatins, and kininogens -- The cystatin superfamily -- Structure: chicken cystatin and human cystatin C -- Cysteine proteinase inhibitors -- Dimerization and domain swapping.

Cystatin C is a mammalian cysteine protease inhibitor, synthesized in various amounts by many kinds of cells and appearing in most body fluids. There are reports that it may be synthesized in the mammalian retina and that a cysteine protease inhibitor may influence the degradation of photoreceptor outer segment proteins.

The cystatin I25 superfamily is a well-established family of cysteine protease inhibitors, which is composed of three distinct subfamilies according to the MEROPS classification (18, 19): I25A (stefins, family 1, type 1, and cystatins A and B), I25B (cystatins, family 2, type 2, and cystatins C, E, and S), and I25C (kininogens, family 3, and.The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions.

The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin .Recombinant Human Cyststatin-C is a bioactive protein intended for use in cell culture applications.

Cystatin-C is a cysteine proteinase inhibitor that is involved in kidney function, cardiovascular disease and neurologic disorders. Cystatin-C has been shown to colocalize with amyloid beta in Alzheimer disease patients.